ABSTRACT. The present study was carried out with the objective of determining the requirements for digestible lysine in Nile tilapia during the 500 to 600 g live weight stage. Isocaloric and isonitrogenous diets were tested with increasing amounts of digestible lysine. L-lysine HCl (78%) were used to replace glutamic acid at increasing levels, resulting in treatments of 9.3, 12.3, 15.3, 18.3 and 21.3 g kg-1 of digestible lysine. Three hundred Nile tilapias with an average weight of 519 ± 27.23 g were used and distributed among 25 tanks. The physical and chemical parameters of the water were pH, dissolved oxygen, salinity, conductivity and temperature. The mortality rate was registered daily. Two slaughters were performed at 28 and 50 days after the beginning of the experiment. It was estimated that an amount of 13.1 g kg-1 of digestible lysine was ideal for obtaining higher WG. Fish slaughtered after 50 days, the digestible lysine requirements were determined to be 14.5 g kg-1 for the lowest carcass humidity and 14.6 g kg-1 for the greatest carcass ethereal extract. Nile tilapias with live weight between 500 and 600 grams require 13.1 g kg-1 of digestible lysine in the diet for greater WG (90.53 g) and better FCR (2.55).

ABSTRACT Growth performance, organ biometrics, bone characteristics, and intestinal morphometry were evaluated in broilers fed a diet containing orange (Citrus sinensis L.) essential oil. A completely randomized design was used, with five treatments with orange essential oil (0, 100, 200, 300, and 400 mg kg−1 diet) and six replications with 20 birds per experimental unit. In the pre-starter phase, feed intake and weight gain of all birds linearly increased, while feed conversion decreased with the addition of orange essential oil in the feed. At day 21, bone density (Seedor Index) and body weight were higher in the birds that received the maximum level of essential oil (400 mg kg−1) compared with those not treated with essential oil. The observed effects resulted from the better functioning of the physiological mechanisms of digestion and absorption of nutrients, characterized by the increase in villus height. Glycemia and weights of gastrointestinal tract organs of broilers at 21 days of age were not influenced by the evaluated essential oil. The results show that the addition of phytogenic additives to the diet does not cause any physiological impairment in birds.

ABSTRACT Two experiments were conducted using direct method to determine the standardized ileal phosphorus (P) digestibility coefficient (SIPDC) of meat and bone meal (MBM) and poultry byproduct meal (PBM) for broilers. In the first experiment, three MBM from different batches were evaluated, with four treatments (MBMI, MBMII, MBMIII, and a P-free diet) and nine replicates of seven birds each, totaling 252 birds distributed across a completely randomized design. The P-free diet was used to determine endogenous P losses. Titanium dioxide (5 g kg−1) was used as an indigestible indicator. In the second experiment, three PBM from different batches were evaluated, with 189 birds distributed over three treatments (PBMI, PBMII, and PBMIII) and nine replicates of seven birds each. These two experiments were conducted when birds were 19-22 days old. The birds were euthanized to collect the ileal content. Using the P-free diet, endogenous P losses were determined at 94.9 mg kg−1 dry matter intake. Standardized ileal P digestibility of MBMI (0.65) and MBMIII (0.69) was higher compared with MBMII (0.49). The SIPDC determined for three PBM sources were similar and 0.96, 0.96, and 0.93 for PBMI, PBMII, and PBMIII, respectively. The present study demonstrates that P in MBM is not as available as it is believed and that the variability between batches should be considered by nutritionists, while PBM can be considered an excellent P source to broiler diets.

ABSTRACT The objective of the present study was to evaluate the effects of xylanase and probiotic supplementation on the performance, carcass characteristics, intestinal pH, intestinal viscosity, and ileal microbiota of broiler chickens fed diets containing wheat bran. The study animals were kept in metal cages, and the study was performed using a completely randomized design, with four treatments, six birds per treatment, and six replicates. The four treatments included a control group, a probiotic-supplemented group, a xylanase-supplemented group, and a group that received both xylanase and probiotic supplementation. The diets of all four groups contained wheat bran (50 and 30 g/kg for the starter and grower phases, respectively) and phytase, and at 10 d after hatching, the experimental birds were challenged orally with Eimeria sp commercial vaccine. During the initial phase, supplementation with xylanase, probiotics, or their combination yielded greater weight gains than the control diet; however, considering the period from 10-35 d, the chickens receiving xylanase + probiotic and the diet without the additives showed lower weight gain (2.746 and 2.600 kg, respectively). All the supplemented diets reduced cecum viscosity, and supplementation with probiotic showed a significantly lower pH (6.11). The ileal microbiota was also influenced by xylanase and probiotic supplementation, modulating the frequencies of the genera Lactobacillus and Clostridium. The positive effects of supplementation with xylanase or probiotics alone were similar to those of co-supplementation, and no associative effect was observed.

Resumo O objetivo nesta pesquisa foi determinar a composição bromatológica e os valores de energia metabolizável para frangos de corte de crescimento lento em diferentes idades dos ingredientes: farelo de algodão, farelo de arroz e farelo de trigo. Para determinação dos valores energéticos foi utilizado o método de coleta total de excretas em dois ensaios de digestibilidade de 15 a 25 e de 35 a 45 dias de idade. As aves foram distribuídas em delineamento inteiramente casualizado, avaliando o efeito da idade, com seis repetições constituídas de 10 aves, para cada ingrediente. Os valores de energia metabolizável aparente corrigida pelo balanço de nitrogênio (kcal/kg), para primeira e segunda idade respectivamente, foram: farelo de algodão, 1.255 e 1.564; farelo de arroz, 3.124 e 3.301; farelo de trigo, 2.211 e 2.383. Os alimentos apresentaram variação quanto a composição química, bem como o valor energético foi superior (P<0,05) para os frangos de maior idade.

Abstract The aim of this study was to evaluate bromatological composition and metabolizable energy values of cottonseed meal, rice meal, and wheat bran when provided to slow growth rate broilers at different ages. Energy values of 15- to 25- and 35- to 45-day-old broilers were determined by the total feces collection method using two digestibility bioassays. The birds were distributed in a completely randomized experimental design to evaluate the age effect, with six replicates of 10 chickens each, for each ingredient. Values of apparent metabolizable energy adjusted by nitrogen balance (kcal/kg), for the first and second ages were: 1,255 and 1,564 for cottonseed meal, 3,124 and 3,301 for rice meal, and, 2,211 and 2,383 for wheat bran, respectively. The ingredients showed some variations regarding chemical composition and energy values, which were higher (P < 0.05) in older broiler chickens.

ABSTRACT Five experiments were conducted to estimate the digestible threonine requirements of slow-growing broilers: experiment 1, starter phase (days 10 to 28); experiment 2, grower I phase (days 29 to 49); experiment 3, grower II phase (days 50 to 69); experiment 4, finisher phase (days 70 to 84); and experiment 5, which was specifically conducted to determine the production of intestinal mucin over two periods (days 50 to 69 and 70 to 84). Different birds were used in all experiments. A completely randomized design with five treatments and four replicates was used. Treatments consisted of increasing the values of digestible threonine in the diet through basal feed supplementation with L-threonine (98.5%), which was added instead of cornstarch. The following values of digestible threonine were investigated: 0.622, 0.697, 0.772, 0.847, and 0.922% in experiment 1; 0.586, 0.662, 0.738, 0.814, and 0.890% in experiment 2; 0.570, 0.640, 0.71, 0.780, and 0.850% in experiments 3 and 5; and 0.520, 0.595, 0.670, 0.745, and 0.820% in experiments 4 and 5. The digestible threonine values presented quadratic effects on feed conversion ratio in experiments 1, 2, and 3. Digestible threonine values of 0.628 and 0.609% resulted in higher villus height and greater duodenal crypt depth, respectively. Digestible threonine values of 0.762, 0.767, and 0.733% may be recommended for the starter, grower I, and grower II phases, respectively, based on the best feed conversion ratio. In addition, a digestible threonine level of 0.694% may be recommended for the finisher phase based on the highest production of intestinal mucin.

ABSTRACT Two experiments were conducted to evaluate the apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), and standardized ileal amino acid digestibility coefficient (SIDC) of extruded soybean cake (ESC) at four different extrusion temperatures: 90, 100, 110, and 120 °C. In experiment 1, 300 male broilers were randomly distributed into four treatments (600 g kg−1 reference diet + 400 g kg−1 of SBC at different extrusion temperatures) + reference diet, with six replicates of 10 birds. A metabolic assay using the total excreta collection method was performed from 14 to 23 days to determine AME and AMEn. Quadractic effect of the extrusion temperature was observed, with the maximum AMEn value estimated as 3424.99 kcal kg−1 for 105.58 °C. In experiment 2, 300 male broilers were randomly distributed into four treatments (453 g kg−1 protein free diet + 547 g kg−1 SBC at different extrusion temperatures) + protein free diet, with six replicates of 10 birds. Experimental diets were offered from 16 to 21 days, and at 21days, birds were sacrificed by cervical dislocation for collection of the ileal digesta to determine the SIDC of ESC. The best SIDC values for lysine (0.824%), methionine (0.908%), and cysteine (0.741%) were estimated at 117.72, 114.40, and 107.37 °C, respectively. The other amino acids evaluated showed a linear response, with better SIDC values with increasing extrusion temperatures. The extrusion temperatures affect metabolizable energy and amino acid digestibility of soybean cake for broilers, being important the standardization of extrusion temperature to maximize the nutritional value of soybean cake used in broiler diets.

RESUMO A determinação da real exigência de lisina é fundamental, por ser um aminoácido essencial e desempenhar importantes funções no metabolismo dos frangos de corte. Além disto, apresenta-se como segundo aminoácido limitante para frangos de corte alimentados com rações formuladas a base de milho e farelo de soja. O objetivo deste estudo foi determinar a exigência de lisina digestível para frangos de corte machos de menor potencial genético para crescimento em sistema semiconfinado, bem como avaliar os efeitos de diferentes valores de lisina digestível sobre o desempenho e características de carcaça. Foram utilizados 300 frangos de corte, da linhagem comercial EMBRAPA 041, com pesoinicial médio de 987 g. Os frangos foram distribuídos em 20 unidades experimentais em um delineamento inteiramente casualizado, com cinco tratamentos (0,586; 0,746; 0,906; 1,066 e 1,226% de lisina digestível), quatro repetições e quinze frangos por unidade experimental. Nos dois períodos avaliados, verificaram-se efeito linear dos níveis de lisina digestível sobre a ingestão e eficiência na utilização de lisina, e efeito quadrático sobre peso vivo final, ganho de peso e a conversão alimentar. Verificou-se efeito quadrático sobre os pesos absolutos da carcaça, peito, coxa+sobrecoxa e rendimento da moela. Podem ser recomendadas, respectivamente, para máximo ganho de peso rações com 1,040% e 1,103% de lisina digestível nos períodos de 35 aos 70 e 35 aos 84 dias de idade.

ABSTRACT Determining the actual lysine requirement is crucial as it is an essential amino acid and plays animportant role in the metabolism of broilers. In addition, it is the second limiting amino acid for broilers fed with rations formulated basedon corn and soybean meal. The aim of this study was to determine the digestible lysine requirement for male broilers with lower genetic potential for growth in a semi-confined system as well as to evaluate the effects of different values of digestible lysine on performance and carcass characteristics. A total of 300 broiler chickensfrom a commercial lineage EMBRAPA 041 were used with an average initial weight of 987 g. The broilers were distributed into 20 experimental units in a completely randomized design with five treatments (0.586; 0.746; 0.906; 1.066, and 1.226% digestible lysine), four replicates and 15 broilers per experimental unit.In both periods, there were linear effect of digestible lysine values on the lysine intake and lysine utilization efficiency and quadratic effect on final body weight, weight gain and feed: gain ratio.There was a quadratic effect on the absolute weight of carcasses, breast, thigh + drumstick and income gizzard.For rearing of male broilers with lesser genetic growth potential reared in semi-confinement, rations with 1.040% and 1.103% digestible lysine can be recommended for maximum weight gain in periods from 35 to 70 and 35 to 84 days of age, respectively.

Objetivou-se avaliar os benefícios da adição da parede celular de Sacharomyces cerevisiae (PCSC) e seu melhor nível de inclusão na dieta sobre o desempenho e a qualidade dos ovos produzidos por codornas japonesas (Coturnix coturnix japonica) na fase de produção. Foram utilizadas 400 codornas japonesas distribuídas em um delineamento inteiramente casualizado, com cinco tratamentos e 10 repetições com 8 aves cada. Foram utilizadas cinco dietas com diferentes níveis de parede celular de S. cerevisiae (0 kg/t; 0.5kg/t; 1.0kg/t; 1.5kg/t; 2.0kg/t). Para análise do desempenho das aves e da qualidade dos ovos, as variáveis avaliadas foram: o consumo de ração, a produção de ovos, o peso médio dos ovos, a massa de ovos, a conversão alimentar por massa, a conversão alimentar por dúzia de ovos produzidos, a viabilidade, a percentagem dos constituintes do ovo (gema, albúmen e casca) e a espessura de casca. O peso dos ovos, a massa de ovos, a viabilidade, a percentagem da gema e a percentagem do albúmen não foram influenciadas pela inclusão de parede celular de S. cerevisiae na dieta das codornas japonesas. A inclusão de níveis crescentes de parede celular de S. cerevisiae até o nível de 1,5kg/t na dieta melhorou a produção, a conversão alimentar por dúzia, a conversão alimentar por massa e a qualidade da casca de ovos produzidos por codornas japonesas durante a fase de produção.

Aimed to evaluate the benefits of adding the cell wall of Saccharomyces cerevisiae (CWSC) and its best level in the diet on the performance and quality of eggs produced by Japanese quail (Coturnix coturnix japonica), during the production phase. 400 Japanese quails, were distributed in a complete random design, with five treatments and 10 replicates with 8 birds each. It was used five diets with different levels of cell wall of S. cerevisiae (0 kg/t; 0.5kg/t; 1.0kg/t; 1.5kg/t; 2.0kg/t). To analyze the performance of birds and egg quality, the variables evaluated were: feed intake, egg production, egg weight, egg mass, feed conversion per dozen of eggs produced, feed conversion per mass of eggs produced, viability, percentage of the yolk, percentage of the albumen, percentage of the shell and shell thickness. Egg weight, percentage of yolk and percentage of albumen were not influenced by the inclusion of cell wall of S. cerevisiae in the diet of Japanese quails. The increasing levels of cell wall of S. cerevisiae to the level of 1.5kg/t in the diet improved production, feed conversion per dozen, feed conversion per mass and shell quality of eggs produced by Japanese quail during the production phase.

O objetivo deste trabalho foi determinar o efeito da piperina, como suplemento fitogênico, na dieta de frangos de corte. Pintos com sete dias de idade foram distribuídos aleatoriamente em quatro tratamentos (n = 24), com quatro repetições (n = 6). A piperina foi adicionada na ração nas concentrações de 0, 60, 120 e 180 mg kg‑1 por 35 dias consecutivos. Foram avaliados: parâmetros bioquímicos, hematológicos e histopatológicos; desempenho e rendimento de carcaça. Também foram realizadas análises histomorfométricas. A adição de 120 e 180 mg kg‑1 de piperina não alterou o peso corpóreo e a conversão alimentar dos frangos, enquanto 60 mg kg‑1 de piperina interferiram positivamente em ambos os parâmetros, de 36 a 42 dias de idade, e aumentaram significativamente a superfície de absorção do duodeno e do íleo. Não foi observada alteração macroscópica no tamanho e na coloração dos órgãos dos frangos alimentados com as rações com as concentrações de piperina avaliadas. A suplementação com 120 e 180 mg kg‑1 de piperina é tóxica ao tecido hepático e reduz a superfície de absorção do jejuno. A dieta suplementada como 60 mg kg‑1 de piperina é segura.

The objective of this work was to determine the effect of piperine as a phytogenic additive in chicken broiler diet. Seven‑day‑old male chicks were randomly allocated in four experimental treatments (n = 24), with four replicates (n = 6). The piperine was added to diets at concentrations of 0, 60, 120, and 180 mg kg‑1 for 35 consecutive days. The following were evaluated: biochemical, hematological and histopathological parameters; performance and carcass yield. Histomorphometric analyses were also carried out. The addition of 120 and 180 mg kg‑1 of piperine did not alter broiler body weight and feed conversion, whereas 60 mg kg‑1 of piperine interfered positively in both parameters from 36 to 42 days of age and significantly increased the absorption surface of the duodenum and the ileum. No macroscopic alteration in organ size and color was observed in the broilers fed diets with the evaluated concentrations of piperine. The supplementation of 120 and 180 mg kg‑1 of piperine is toxic to liver tissue and reduces the absorption surface of the jejune. The diet supplemented with 60 mg kg‑1 of piperine is safe.

Um experimento foi realizado para avaliar a influência da forma física da ração sobre o desempenho e as características da carcaça de frangos de corte. Foram utilizados 240 pintos de corte machos, distribuídos e alojados em baterias metálicas de três andares em delineamento de blocos ao acaso com 4 formas físicas de ração e 6 repetições com 10 aves por unidade experimental, considerando cada andar um bloco. Os tratamentos consistiram em rações farelada, peletizada, expandida e expandida peletizada. Os índices de desempenho foram analisados no período de 1 a 22 e 1 a 39 dias de idade das aves. Duas aves com peso médio do grupo foram retiradas de cada repetição e abatidas aos 40 dias de idade para avaliação da carcaça. A forma física da ração afetou o desempenho e as características da carcaça dos frangos. Nos períodos de 1 a 22 dias e de 1 a 39 dias, os frangos que consumiram o alimento farelado apresentaram menor consumo de ração, peso médio e ganho de peso, enquanto aqueles que receberam ração peletizada não apresentaram diferença no peso médio, ganho de peso e conversão alimentar em relação àqueles que receberam ração expandida peletizada. Os maiores resultados para essas variáveis foram observados para aves alimentadas com ração peletizada ou expandida peletizada. As aves que receberam ração expandida peletizada apresentaram melhores resultados de peso de carcaça e dos cortes em relação àquelas que receberam a ração farelada e este melhor resultado não diferiu do encontrado para as aves alimentadas com ração peletizada. Recomenda-se o uso de rações na forma física peletizada ou expandida peletizada, pois promoveram os melhores índices zootécnicos.

An experiment was conducted to evaluate the influence of the physical form of feed on performance and carcass characteristics of broilers. A total of 240 male broiler chicks were assigned and housed in metal cage of three floors in a randomized block design with 4 physical forms of feed and 6 repetitions with 10 birds per experimental unit, where each floor represented a block. Treatments consisted of: mash, pelleted, expanded and expanded pelleted diets. Performance indexes were analyzed in the period from 1 to 22 and 1 to 39 days of birds' life. Two birds with an average weight of the group were taken from each repetition and slaughtered at 40 days of age for carcass evaluation. The physical form of ration affected the performance and carcass characteristics of broilers. For the period from 1 to 22 days and from 1 to 39 days, chickens that consumed the mashed food had lower feed intake, weight and weight gain, whereas birds fed pelleted diets showed no difference in weight, weight gain or feed conversion from those fed expanded pelleted diets. The major results for these variables were observed for birds fed pelleted or expanded pelleted diets. Birds fed expanded pelleted diets showed the best results for carcass and cut weight compared with those fed with mesh diet, and this better outcome did not differ from that found for birds fed pelleted diet. The use of rations in pelleted or expanded pelleted physical forms is recommended, for they promote the best animal performance parameters.

Com o objetivo de avaliar níveis de metionina + cistina para matrizes pesadas de 40 a 60 semanas de idade, conduziu-se um experimento com 288 matrizes da linhagem Cobb-500, distribuídas em delineamento inteiramente casualizado, com seis níveis de metionina + cistina nas dietas (0,39; 0,47; 0,55; 0,63; 0,71 e 0,79%), oito repetições e seis matrizes por unidade experimental. As rações foram isocalóricas e isoproteicas (2.850 kcal EM/kg e 12,58% PB) e fornecidas em quantidade fixa (155 g) por ave. No período total estudado, por meio de análise polinomial, foi observado efeito quadrático dos níveis de metionina + cistina sobre o percentual de produção de ovos, o número de ovos por ave alojada, o número de ovos por ave e o peso dos ovos. Houve também efeito linear sobre o peso da casca quando calculado em relação ao peso dos ovos. A exigência de metionina + cistina total para matrizes pesadas foi estimada em 0,521% para percentual de produção de ovos e número de ovos por ave, o que corresponde a uma ingestão diária de 808 mg de metionina + cistina total. A exigência de metionina + cistina digestível para matrizes pesadas é de 727 mg para percentual de postura e número de ovos por ave.

The objective of this experiment was to evaluate levels of methionine + cystine levels for broiler breeders from 40 to 60 weeks of age. It was used 288 Cobb broiler breeders, distributed in a randomized experimental design with six levels of methionine+ cystine in the diets (0.39; 0.47; 0.55; 0.63; 0.71 and 0.79%), eight repetitions and six breeders per experimental unit. The diets were isocaloric and isoprotein (2,850 kcal ME/kg and 12.58% CP) and oferred at a fixed amount (155 g) per bird. For the entire studied period, by using polynomial analysis, it was observed a quadratic effect of methionine + cystine levels on the percentage of egg production, on number of eggs per hen housed, on number of eggs per hen and egg weight. There was also a linear effect on shell weight when it was calculated in relation to egg weight. The total methionine + cystine requirement for broiler breeders was calculated at 0.521% for percentage of egg production and number of eggs per hen, which corresponds to a daily intake of 808 mg of methionine + cystine/day. The digestible methionine + cystine requirement for broiler breeders was estimated at 727 mg for laying percentage and number of eggs per hen.

O efeito tóxico da piperina, principal amida presente em diversas espécies de pimenta, foi avaliada em frangos de corte por meio da administração oral (0,00, 1,12, 2,25 e 4,5mg kg-1 peso vivo) por 14 dias consecutivos. Foram empregados 60 pintos machos (Cobb Avian 48) com sete dias de idade, distribuídos aleatoriamente em quatro grupos experimentais (n=15). Foram avaliados parâmetros como: ganho de peso, peso relativo do fígado e alterações hematológicas e anatomopatológicas. A administração oral de piperina não interferiu no ganho de peso ou no peso relativo do fígado, além de não promover alteração no tamanho e na coloração dos órgãos ou no aparecimento de lesões de parênquima e nas mucosas do órgão. Todavia, alterações histopatológicas dose-dependentes foram observadas. A piperina não foi capaz de alterar significativamente os parâmetros hematológicos analisados, com exceção do leucograma, em que as doses de 1,12mg e 2,25mg kg-1 promoveram aumento do número de heterófilos e do número total de leucócitos, respectivamente. Os resultados sugerem que a dose oral de 1,12mg de piperina por quilo não é tóxica para frangos de corte, sendo semelhante aos resultados obtidos para ratos e camundongos. Além disso, constatou-se a capacidade da piperina em aumentar o número total de leucócitos circulantes a partir da dose de 2,25mg kg-1 nessa espécie animal.

The toxic effect of piperine, the main amide compound of different pepper species, was evaluated on broiler chickens by oral administration at 0.00, 1.12, 2.25 and 4.50mg kg-1 concentrations for 14 days. Sixty seven days old male chicks (Cobb Avian 48) randomly allocated to four experimental groups (n=15) were used in this work. Parameters such as: body weight gain, liver relative weight, hematological and anatomopathological alterations were analyzed. The oral route administration did not interfere on weight gain or liver relative weight, as well as, any modification on size and organs' color and/or parenchyma/mucous membranes injuries were observed; however, liver histopathological changes were noticed in a dose-dependent manner. In addition, piperine did not alter hematological parameters, except for leukocytes counting, which at 1.12 and from 2.25mg kg-1 caused an increase of heterophils and in the total number of leukocytes, respectively. The results suggest that 1.12mg kg-1 of piperine orally administrated is not toxic for broiler chickens, as previously observed for rats and mice. Moreover, 2.25mg kg-1 of piperine seems to increase the total number of leukocytes for this animal specie.

Dois experimentos, utilizando pintos de corte Avian Farm, no período de 8 a 18 dias de idade, foram realizados para se avaliar o efeito de enzimas microbianas sobre os coeficientes de digestibilidade (CD) ileal aparente da matéria seca (MS), da proteína bruta (PB), da energia bruta (EB), do fósforo (P) e do cálcio (Ca) de dietas à base de milho e farelo de soja com diferentes níveis de Ca e P disponível (Pd). Óxido crômico (0,5%) foi adicionado às dietas a fim de se estimar o fator de indigestibilidade. Todas as aves foram abatidas aos 19 dias, para a coleta da digesta. O primeiro experimento foi realizado em arranjo fatorial 2 x 2, com seis repetições contendo 10 aves. As dietas foram formuladas contendo dois níveis de Ca e Pd normal (1%Ca/0,45%Pd) e baixo (0,70%Ca/0,32%Pd) x dois níveis de enzima fitase (0 e 1 kg/t de ração). O segundo experimento foi realizado em arranjo fatorial 2 x 3, sendo dois níveis de Ca e Pd normal (1%Ca/0,45%Pd) e baixo (0,70%Ca/0,32Pd%) e três combinações de enzimas: complexo multienzimático (CM) com fitase (Fit): 0 kg/t para o controle, 2 kg/t (CM) e 2 kg/t mais 1 kg/t de fitase (CM+Fit), com seis repetições, contendo 10 aves cada. No experimento 1, a adição de fitase melhorou a digestibilidade ileal da MS (5,2%), PB (2,4%) e EB (3,8%). Interação significativa foi observada na digestibilidade do Ca e P, sendo que adição da fitase melhorou a digestibilidade do Ca e P em ambos níveis de Ca e P. No segundo experimento, a adição do CM melhorou o CD da PB, P e Ca em 3; 4,7; e 7,8%, respectivamente. A associação do CM+fitase mostrou efeito aditivo no aproveitamento do Ca e P. Interação foi observada para o CD da EB, a adição do CM melhorou a digestibilidade em ambos níveis de Ca e Pd, entretanto o CM+fitase melhorou o CD da EB apenas no nível baixo de Ca e P.

Two experiments, using 8 to 18 days old Avian -Farm male broiler chicks, were conducted to evaluate the effect of microbial enzymes on the ileal digestibility coefficient (DC) of dry mater (DM), crude protein (CP), energy (E), calcium (Ca) and phosphorus (P) in corn soybean meal diets with different level of Ca and available P (Pa). Chromic oxide (0,5%) was added to the diets, as an indigestible marker, to estimate ileal digestibility. All chicks were killed at the end of the experiments (19 days) to collect the ileal content. The first experiment was a factorial arrangement of 2 x 2, with six replicates (n=240, 10 chicks per unit). Diets were formulated to contain two levels of Ca and Pa normal (1%Ca/0.45%Pa) and low (0.70%Ca/0.32%Pa) x two level of phytase (Phy) enzyme (0 and 1 kg/t of diet). The second experiment was a factorial arrangement of 2 x 3, six replicates (n=360, 10 chicks per unit). Diets were formulated with two levels of Ca and Pa normal (1%Ca/0.45%Pa) and low (0.70%Ca/0.32%Pa) x three combination of a multienzyme complex (MC). The combination were 0 kg/t for the control (C), 2 kg/t (MC) and 2 kg/t of the MC plus 1 kg/t of phy (MC+phy). In the first experiment the diets with Phy had a higher digestibility for DM (5.2%), CP (2.4%) and GE (3,8%). Phytase improve the digestibility of P and Ca in both levels of Ca and Pa. In the second experiment, the addition of the MC improved DC of CP, P and Ca in 3%; 4.7 and 7.8%, respectively. The association of MC + Phy improved Ca and P digestibility. Interaction was observed in the energy digestibility. The addition of the MC improved the digestibility in both Ca and P levels, however the addition of the CM+Phy improved energy DC only in the diets with low Ca and P levels.